DNA isolation / purification Cells Primary cells

Get tips on using TaqMan™ Fast Universal PCR Master Mix (2X), no AmpErase™ UNG to perform PCR Multiplex PCR - Mammalian DNA

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

Get tips on using QIAprep Spin Miniprep Kit to perform Plasmid Isolation Salmonella enterica serovar Indiana (S. Indiana)

Get tips on using NucleoBond® Xtra Midi / Maxi to perform Plasmid Isolation Shiga toxin-producing E. coli

Get tips on using QIAGEN Plasmid Plus 96 Miniprep Kit (4) to perform Plasmid Isolation E. coli DH5α

Get tips on using B-PER™ Bacterial Protein Extraction Reagent to perform Protein isolation Bacteria - Listeria monocytogenes

Get tips on using B-PER™ Bacterial Protein Extraction Reagent to perform Protein isolation Bacteria - Streptococcus mutans

Get tips on using B-PER™ Bacterial Protein Extraction Reagent to perform Protein isolation Bacteria - Lactobacillus casei

Get tips on using Y-PER™ Yeast Protein Extraction Reagent to perform Protein isolation Yeast - Candida tenuis