Protein expression and purification Mammalian cells

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Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Bacteria - Gram negative Klebsiella pneumoniae

Products Thermo Fisher Scientific mirVana™ miRNA Isolation Kit, with phenol

Get tips on using mirVana™ miRNA Isolation Kit, with phenol to perform RNA isolation / purification Bacteria - Gram negative Salmonella enterica

Products Thermo Fisher Scientific mirVana™ miRNA Isolation Kit, with phenol

Get tips on using MagMAX™ Total Nucleic Acid Isolation Kit to perform DNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis

Products Thermo Fisher Scientific MagMAX™ Total Nucleic Acid Isolation Kit

Get tips on using MagMAX™ Total Nucleic Acid Isolation Kit to perform RNA isolation / purification Bacteria - Gram positive Mycobacterium tuberculosis

Products Thermo Fisher Scientific MagMAX™ Total Nucleic Acid Isolation Kit

Is a knockdown using shRNA permanent and if not is there a known duration?

Discussions Is a knockdown using shRNA permanent?

Get tips on using MagNA Pure Compact Nucleic Acid Isolation Kit I to perform DNA isolation / purification Bacteria - Gram negative Enterobacteriaceae

Products Roche Lifesciences MagNA Pure Compact Nucleic Acid Isolation Kit I

Get tips on using PowerSoil® DNA isolation - DNeasy PowerSoil Pro Kit to perform DNA isolation / purification Bacteria - Gram negative Enterobacteriaceae

Products Qiagen PowerSoil® DNA isolation - DNeasy PowerSoil Pro Kit

There are a plethora of detection methods of cell cytotoxicity and proliferation by flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Cellular assays Cell cytotoxicity / Proliferation assay cell type MCF-7

Get tips on using Easy-Spin (DNA free) Total RNA Extraction Kit to perform RNA isolation / purification Bacteria - Gram negative Vibro parahaemolyticus

Products iNtRON Biotechnology Easy-Spin (DNA free) Total RNA Extraction Kit

Get tips on using TRIzol™ Max™ Bacterial RNA Isolation Kit to perform RNA isolation / purification Bacteria - Gram negative Vibro parahaemolyticus

Products Thermo Fisher Scientific TRIzol™ Max™ Bacterial RNA Isolation Kit

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