Wound healing assay cell type human

Get tips on using MitoSOX™ Red Mitochondrial Superoxide Indicator, for live-cell imaging to perform ROS assay cell type - PC-3 human prostate adenocarcinoma

Get tips on using APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU to perform TUNEL assay cell type - A549, NCI-H460, H1299 human alveolar carcinoma

Get tips on using In Situ Cell Death Detection Kit, TMR red to perform TUNEL assay cell type - A549, NCI-H460, H1299 human lung cancer cells

Get tips on using Autophagy Assay Kit to perform Autophagy assay cell type - Beas-2B

Get tips on using Autophagy Assay Kit to perform Autophagy assay cell type - Mouse cardiomyocytes

Get tips on using Cytoselect™ Cell Viability and Cytotoxicity Assay to perform Live / Dead assay mammalian cells - MCF-7 human breast cancer cells

Get tips on using CytoScan™ SRB Cytotoxicity Assay to perform Cell cytotoxicity / Proliferation assay cell type - CHO-K1

Get tips on using LDH-Cytotoxicity Colorimetric Assay Kit to perform Cell cytotoxicity / Proliferation assay cell type - SH-SY5Y

Get tips on using Cytotoxicity LDH Assay Kit-WST to perform Cell cytotoxicity / Proliferation assay cell type - L-02

An alternative to culture-based cell death detection is an assessment of other cell viability indicators using fluorescent dyes, including membrane potential and membrane integrity. Live/Dead assays differentiates live and dead cells using membrane integrity as a proxy for cell viability and are based on a fluorescent staining procedure followed by detection using flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.