siRNA / miRNA gene silencing Mouse C2C12

Get tips on using Mm_Pttg1ip_7 FlexiTube siRNA to perform siRNA / miRNA gene silencing Mouse - AtT20 PTTG1

Get tips on using Mm_Gadd45b_4 FlexiTube siRNA to perform siRNA / miRNA gene silencing Mouse - AtT20 GADD45β

Get tips on using Cdx2 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - mESC Cdx2

Get tips on using BECN1 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - BV2 BECN1

Get tips on using CD98 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - Colon26 CD98

Get tips on using CD98 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - RAW264.7 CD98

Get tips on using Mm_Hdac5_2 FlexiTube siRNA to perform siRNA / miRNA gene silencing Mouse - RAW264.7 HDAC5

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using Cxcr4 siRNA to perform siRNA / miRNA gene silencing Mouse - Embryonic stem cells CXCR4