Protein Expression Prokaryotic cells B. bifidum

Get tips on using Bax Antibody (4H32): sc-70407 to perform Western blotting Bax

Get tips on using BECN1 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - BV2 BECN1

Get tips on using TGF-beta Pan Specific Antibody to perform Western blotting TGF-beta1

Get tips on using Anti-Bcl-2 antibody (ab196495) to perform Western blotting Bcl- 2

Get tips on using Recombinant Anti-Bax antibody [E63] (ab32503) to perform Western blotting Bax

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using QIAamp BiOstic Bacteremia DNA Kit (50) to perform DNA isolation / purification Fish

Get tips on using Bcl-2 Antibody (100): sc-509 to perform Western blotting Bcl- 2

Get tips on using Bacteria Live/Dead Staining Kit to perform Live / Dead assay bacteria - Bacillus subtilis