Protein Expression Prokaryotic cells L. lactis

Get tips on using Lamin A/C Monoclonal Antibody (mab636) to perform Western blotting Lamin A/C

Get tips on using ON-TARGETplus Mouse Lgals3 siRNA to perform siRNA / miRNA gene silencing Mouse - BV2 LGAL3S3

Get tips on using Lipofectamine® 2000 Transfection Reagent to perform siRNA / RNAi /miRNA transfection Rat - C6 Lipofectamine

Get tips on using LIVE/DEAD™ Yeast Viability Kit to perform Live / Dead assay yeast - Urediniospore

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using E-Gel™ 50 bp DNA Ladder to perform DNA Ladder 50 bp

Get tips on using TriDye™ 1 kb Plus DNA Ladder to perform DNA Ladder 1 kb

Get tips on using TrackIt™ 1 Kb Plus DNA Ladder to perform DNA Ladder 1 kb

Get tips on using Lamin B1 Antibody (C-5): sc-365962 to perform Western blotting Lamin B

Get tips on using Mouse Lipocalin-2 ELISA Kit (NGAL) (ab199083) to perform ELISA Mouse - NGAL/LCN2