siRNA / miRNA gene silencing Human EM-2

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Get tips on using MLM3636 to perform CRISPR Mouse - Deletion Neuro 2a Prnp

Get tips on using JDS246 to perform CRISPR Mouse - Deletion Neuro 2a Prnp

Get tips on using lentiCRISPR to perform CRISPR Mouse - Deletion RAW 264.7 Tfeb

Get tips on using Acridine Orange solution to perform Necrosis HT-29

Get tips on using LC3B Antibody to perform Autophagy assay cell type - RAW 264.7

Get tips on using CAG-Cas9 to perform CRISPR Mouse - Deletion Neuro 2a Rab38

Get tips on using lentiCas9-Blast to perform CRISPR Mouse - Deletion RAW 264.7 Casp1

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized MDAH 2774

Get tips on using RNA-Bee to perform RNA isolation / purification Cells - immortalized MDAH 2774