Protein expression and purification

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using EZ DNA Methylation-Gold Kit to perform DNA methylation profiling Gene specific profiling - HepG2 Constitutive Androstane Receptor (CAR)

Get tips on using Q5® Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Human - Point mutation LNCaP Androgen Receptor splice variant (AR-V)

Get tips on using Annexin V / 7-AAD to perform Apoptosis assay cell type - MCF-7

Get tips on using FITC Annexin V Apoptosis Detection Kit with 7-AAD to perform Apoptosis assay cell type - SKOV3

Get tips on using FITC Annexin V Apoptosis Detection Kit with 7-AAD to perform Apoptosis assay cell type - PANC-1

Get tips on using PE Annexin V Apoptosis Detection Kit with 7-AAD to perform Apoptosis assay cell type - Human T-cells

Get tips on using PE Annexin V Apoptosis Detection Kit with 7-AAD to perform Apoptosis assay cell type - T-cells Mouse (OT-I)

Get tips on using CD73 Monoclonal Antibody (AD2), PE, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD73

Get tips on using CD73 Monoclonal Antibody (AD2), APC, eBioscience™ to perform Flow cytometry Anti-bodies Mouse - CD73