siRNA / miRNA gene silencing Human HNSCC cell line Eph receptor B4

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using Corning® BioCoat™ Matrigel® Invasion Chambers with 8.0 µm PET Membrane in two 24-well Plates to perform Cell migration / Invasion cell type - BxPC-3

Get tips on using Corning® BioCoat™ Matrigel® Invasion Chambers with 8.0 µm PET Membrane in two 24-well Plates to perform Cell migration / Invasion cell type - MG-63

Get tips on using pEV-UDP-E to perform Protein Expression Prokaryotic cells - E. coli 2-epimerase

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rat epidermal keratinocytes

Get tips on using QuantiFluor® dsDNA System to perform DNA quantification MCF10A breast epithelial cells

Get tips on using Qubit dsDNA HS Assay Kit to perform DNA quantification MCF10A breast epithelial cells

Get tips on using Phusion Site-Directed Mutagenesis Kit to perform Site Directed Mutagenesis (SDM) Human - Deletion MDA-MB-231 sodium channel β1 subunit

Get tips on using QuikChange Site-Directed Mutagenesis Kit, 10 Rxn to perform Site Directed Mutagenesis (SDM) Human - Point mutation A549 β-arrestin-2

Get tips on using QuikChange Site-Directed Mutagenesis Kit, 10 Rxn to perform Site Directed Mutagenesis (SDM) Human - Point mutation MDA-MB-231 CD44