rna-isolation-purification-cells-primary-rat-cardiac-fibroblasts

Get tips on using Gibco™StemPro™ Adipogenesis Differentiation Kit to perform Stem cell Differentiation media hMSCs differentiation into adipogenic cells

Get tips on using Gibco™StemPro™ Chondrogenesis Differentiation Kit to perform Stem cell Differentiation media hBMSCs differentiation into chondrogenic cells

Get tips on using ApopTag Fluorescein in Situ Apoptosis Detection Kit to perform TUNEL assay cell type - HEK293 human embryonic kidney cells

Get tips on using TdT In Situ Apoptosis Detection Kit - Fluorescein to perform TUNEL assay cell type - HeLa cells human cervical cancer

Get tips on using Gibco™ DMEM, high glucose, GlutaMAX™ Supplement to perform Stem cell culture media Mouse muscle satellite cells

Get tips on using ROS-ID® Total ROS/Superoxide detection kit to perform ROS assay cell type - K562 human leukemia cells

Get tips on using OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence) to perform ROS assay cell type - K562 human leukemia cells

Get tips on using Purified Mouse Anti-Nucleoporin p62 Clone 53/Nucleoporin p62 to perform Autophagy assay cell type - N27 dopaminergic cells

Get tips on using PE Annexin V Apoptosis Detection Kit with 7-AAD to perform Apoptosis assay cell type - Human T-cells

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.