siRNA / RNAi /miRNA transfection Human Cells THP-1

Get tips on using KAPA RNA HyperPrep Kit with RiboErase (HMR) to perform RNA sequencing Mouse - ESCs (Embryonic Stem Cells)

Get tips on using VWR Life Science RiboZol™ RNA Extraction Reagent to perform RNA isolation / purification Cells - immortalized C2C12

Get tips on using Quant-iT™ RiboGreen™ RNA Assay Kit to perform RNA quantification Fuorimetric - mouse blood cells

Get tips on using Quant-iT™ RiboGreen™ RNA Assay Kit to perform RNA quantification Fuorimetric - mouse glial cells

Get tips on using Gibco™DMEM/F-12 to perform Stem cell Differentiation media hESCs or iPSCs differentiation into ovarian follicle/granulosa cells

Get tips on using Gibco™Advanced DMEM/F-12 to perform Stem cell Differentiation media hPSCs or iPSCs differentiation into Lung progenitor cells

The RNA-guided CRISPR-Cas9 nuclease system has revolutionized the genome editing practices. For the most part, the Cas9-mediated genome editing is performed either via nonhomologous end joining (NHEJ) or homology-directed repair (HDR) in mammalian cells, However, designing of specific sgRNAs and minimizing off-target cleavage mediated mutagenesis are the major challenges in CRISPR-Cas based genome editing. To circumvent these issues, we can take advantages of many available tools and approaches for sgRNA construction and delivery.

Get tips on using CellTiter-Glo® Luminescent Cell Viability Assay to perform Live / Dead assay mammalian cells - H9C2

Get tips on using CellTiter-Glo® Luminescent Cell Viability Assay to perform Live / Dead assay mammalian cells - MMQ

Get tips on using CellTiter-Glo® Luminescent Cell Viability Assay to perform Live / Dead assay mammalian cells - GH3