siRNA / miRNA gene silencing Rat INS-1

Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized PNT2

Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized LNCaP

Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized DU145

Get tips on using Anti-MMP13 antibody (ab39012) to perform Western blotting MMP-13

Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized U-251

Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized PC-3

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Get tips on using JNK1 Antibody (F-3) to perform Autophagy assay cell type - PC-12

Get tips on using PI/RNase Staining Buffer to perform Cell cycle assay human - HCT-116

Get tips on using APC BrdU Flow Kit to perform Cell cycle assay human - MCF 10A