RNA isolation / purification Cells primary

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rat osteoblasts

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary rat astrocytes

Get tips on using miRNeasy Mini kit to perform RNA isolation / purification Cells - primary hematopoietic stem cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rat brain microvascular endothelial cells

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - primary rabbit aortic smooth muscle cells

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human preadipocytes

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human osteoblasts

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human chondrocytes

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - primary human keratinocytes

Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA