CRISPR Rat Deletion INS-1 832/13

Get tips on using CD49f (Integrin alpha 6) Monoclonal Antibody (eBioGoH3 (GoH3)), eFluor 450, eBioscience™ to perform Flow cytometry Anti-bodies Human - CD49f/ITGA6

Get tips on using INTRAY® COLOREX™ VRE - PREPARED PLATED CULTURE MEDIA FOR VANCOMYCIN RESISTANT ENTEROCOCCI to perform Bacterial cell culture media Enterococcus faecium

Get tips on using INTRAY® COLOREX™ VRE - PREPARED PLATED CULTURE MEDIA FOR VANCOMYCIN RESISTANT ENTEROCOCCI to perform Bacterial cell culture media Enterococcus faecalis

Get tips on using MitoSOX™ Red Mitochondrial Superoxide Indicator, for live-cell imaging to perform ROS assay cell type - A549 human adenocarcinomic human alveolar basal epithelial cells

Get tips on using LC3B antibody to perform Autophagy assay cell type - RT4

Get tips on using CytoSelect™ 24-Well Wound Healing Assay to perform Wound healing assay cell type - human RT-7

I used a serum/plasma kit for my serum samples. After the phase separation the samples should have 3 phases: a colourless aqueous phase, a white interphase and a red organic phase. However, in some of my samples there was no aqueous phase unless I wait for an extended period of time. How can I circumvent this problem?

I would like to regulate the expression of a gene and in order to do that, I have purchased specific siRNA. After optimizing my transfection protocol and using electroporation I have achieved a 60-70% reduction of the gene of interest. However, I cannot observe a significant reduction of mRNA expression but only a reduction of protein. What might be the problem? Could the problem be in my cell treatment method?

What is the optimal concentration for primers in qPCR? My total volume is 20μl per reaction.

Get tips on using p83Xi to perform Protein Expression Eukaryotic cells - S. cerevisiae Integral membrane proteins (IMPs)