RNA isolation / purification Tissue Mouse

DNA ladder is typically used as a reference to estimate the size of unknown DNA samples that are separated based on their mobility in an electrical field. The critical points for running a DNA ladder are compatibility with running buffer, agarose gel percentage, and choosing the correct range of DNA ladder for sizing DNA molecules.

DNA ladder is typically used as a reference to estimate the size of unknown DNA samples that are separated based on their mobility in an electrical field. The critical points for running a DNA ladder are compatibility with running buffer, agarose gel percentage, and choosing the correct range of DNA ladder for sizing DNA molecules.

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.

Get tips on using Unstained Protein Ladder, Broad Range Ladder to perform Protein Ladder Unstained

Get tips on using PageRuler™ Unstained High Range Protein Ladder to perform Protein Ladder Unstained

Get tips on using PageRuler™ Unstained Broad Range Protein Ladder to perform Protein Ladder Unstained

Get tips on using PageRuler™ Unstained Low Range Protein Ladder to perform Protein Ladder Unstained

Get tips on using Spectra™ Multicolor High Range Protein Ladder to perform Protein Ladder Prestained

Get tips on using Spectra™ Multicolor Low Range Protein Ladder to perform Protein Ladder Prestained

Get tips on using Unstained Protein Standard, Broad Range (10-200 kDa) to perform Protein Ladder Unstained