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Get tips on using PI/RNase Staining Buffer to perform Cell cycle assay human - Jurkat

Get tips on using PI/RNase Staining Buffer to perform Cell cycle assay human - A549

Get tips on using PI/RNase Staining Buffer to perform Cell cycle assay human - HCT-116

Get tips on using FxCycle™ PI/RNase Staining Solution to perform Cell cycle assay human - SW480

Get tips on using FxCycle™ PI/RNase Staining Solution to perform Cell cycle assay human - U266

Get tips on using FxCycle™ PI/RNase Staining Solution to perform Cell cycle assay human - HCT-116

Get tips on using FxCycle™ PI/RNase Staining Solution to perform Cell cycle assay human - HL-60

Get tips on using FxCycle™ PI/RNase Staining Solution to perform Cell cycle assay human - THP-1

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.