Protein Expression Eukaryotic cells T. thermophila

Get tips on using Anti-LC3 pAb to perform Autophagy assay cell type - HEK 293

Get tips on using LC3B Rabbit Antibody to perform Autophagy assay cell type - HEK 293

Get tips on using LC3B Rabbit Antibody to perform Autophagy assay cell type - SH-SY5Y

Get tips on using Annexin V-FITC to perform Apoptosis assay cell type - PC-3

Get tips on using Fluoro hROS to perform ROS assay cell type - MiaPaCa-2 pancreatic carcinoma

Get tips on using IBI’s Todd-Hewitt Broth to perform Bacterial cell culture media Salmonella Typhi

Get tips on using Thioglycolate Broth with Resazurine to perform Bacterial cell culture media Clostridium tetani

Get tips on using monoclonal antibody to LC3 to perform Autophagy assay cell type - IEC-6

Get tips on using bisBenzimide H 33342 trihydrochloride to perform Apoptosis assay cell type - OVCAR-3

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.