rna-isolation-purification-tissue-mouse-mammary-glands

Get tips on using Bovine Endothelial Cell Media to perform Mammalian cell culture media BAOEC

Get tips on using MEM (Eagle) Liquid Medium to perform Mammalian cell culture media MDCK

Get tips on using Minimum Essential Media (MEM) to perform Mammalian cell culture media MDCK

Get tips on using Minimum Essential Media (MEM) to perform Mammalian cell culture media Vero

Get tips on using Minimum Essential Medium Eagle to perform Mammalian cell culture media Vero

Get tips on using 2-D Quant Kit to perform Protein quantification Mammalian cells - SiHa

Get tips on using QIAGEN Multiplex PCR Kit to perform PCR Multiplex PCR - Mammalian DNA

Get tips on using jetPEI®-HUVEC to perform DNA transfection Mammalian cells - Primary cells HUVEC

Get tips on using PolyMag Neo to perform DNA transfection Mammalian cells - Immortalized cell lines SH-SY5Y

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.