rna-isolation-purification-tissue-mouse-small-intestine

Get tips on using PI/RNase Staining Buffer to perform Cell cycle assay human - Jurkat

Get tips on using PI/RNase Staining Buffer to perform Cell cycle assay human - A549

Get tips on using PI/RNase Staining Buffer to perform Cell cycle assay human - HCT-116

Get tips on using FxCycle™ PI/RNase Staining Solution to perform Cell cycle assay human - SW480

Get tips on using FxCycle™ PI/RNase Staining Solution to perform Cell cycle assay human - U266

Get tips on using FxCycle™ PI/RNase Staining Solution to perform Cell cycle assay human - HCT-116

Get tips on using FxCycle™ PI/RNase Staining Solution to perform Cell cycle assay human - HL-60

Get tips on using FxCycle™ PI/RNase Staining Solution to perform Cell cycle assay human - THP-1

In ChIP, the most vital step is the binding of an antibody and choosing the right antibody. The binding affinity of different types of immunoglobulins to protein A or G differs significantly. Henceforth, it is recommended to choose either protein A or protein G coated beads. If you do not see any product in the positive control, add 5–10 μg of chromatin and 1–5 μg of antibody to each IP reaction and incubate with antibody overnight and an additional 2 hr after adding Protein G/A beads. If no product is observed in the experimental sample, add more DNA to the PCR reaction or increase the number of amplification cycles. Furthermore, if you have any problem with antibodies, make sure to use the ChIP-validated antibody.

Get tips on using pSpCas9(BB)-2A-GFP (PX458) to perform CRISPR Human - Deletion RNase L