Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized PNT2
Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized LNCaP
Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized DU145
Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized U-251
Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized PC-3
Get tips on using RNAzol® RT to perform RNA isolation / purification Cells - immortalized A-172
Get tips on using Non-phospho (Active) β-Catenin (Ser33/37/Thr41) (D13A1) Rabbit mAb #8814 to perform Immunohistochemistry Mouse - β-Catenin
Get tips on using DYKDDDDK Tag (D6W5B) Rabbit mAb (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #14793 to perform ChIP Anti-bodies FLAG
The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.
The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.
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