PCR ORNi-PCR

Get tips on using PhosphoProtein Purification Kit (6) to perform Protein tag Purification of phosphorylated proteins

Get tips on using pTRAkc-rbcs1-cTP/pRIC 3.0 to perform Protein Expression Prokaryotic cells - A. tumefaciens BFDV cp

Get tips on using Gentra Puregene Cell Kit to perform DNA isolation / purification Cells - Primary cells Human primary keratinocytes

Get tips on using Gyros IncSupplier Diversity Partner REXXIP HN BUFFER 25 ML PER VI DFS Item to perform Protein isolation Bacteria - Borrelia burgdorferi

Get tips on using Wizard® Genomic DNA Purification Kit to perform DNA isolation / purification Cells - Primary cells Human primary keratinocytes

Get tips on using pCas9_GFP to perform CRISPR Mouse - Deletion ANS4 Rosa26

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

Get tips on using pTRK1061 to perform Protein Expression Prokaryotic cells - L. lactis Cry5B

Get tips on using Purified anti-Tubulin β-3 (TUBB3) Antibody (Previously Covance catalog# PRB-435P) to perform Immunohistochemistry Mouse - TUBB3

Get tips on using PureLink™ HiPure Plasmid Filter Purification Kits - for Midi and Maxi preparation of Plasmid DNA to perform Plasmid Isolation Citrobacter koseri