rna-sequencing-mouse-3t3-l1

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Mouse Heart

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Hippocampus

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Eye

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Colon

Get tips on using SV Total RNA Isolation System to perform RNA isolation / purification Tissue - Mouse Adipose

Get tips on using PureLink™ RNA Mini Kit to perform RNA isolation / purification Tissue - Mouse Adipose

Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - mouse kidney tissue

Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - mouse liver tissue

Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - mouse adipose tissue

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.