siRNA / miRNA gene silencing Rat RBL-2H3

When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.

As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.

Get tips on using Fenozol to perform siRNA / miRNA gene silencing Rat - IEC-6 HuR

Get tips on using Slc1a2 to perform siRNA / miRNA gene silencing Rat - Glial cells GLT-1

Get tips on using YAP siRNA (h) to perform siRNA / miRNA gene silencing Human - OV2008 Yap Gene

Get tips on using p130 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - mESC Rbl2/p130

Get tips on using siRNA RBM3 to perform siRNA / miRNA gene silencing Human - PANC-1 RBM3

Get tips on using siRNA RBM3 to perform siRNA / miRNA gene silencing Human - BxPC-3 RBM3

Get tips on using RAB8A siRNA to perform siRNA / miRNA gene silencing Human - HEK293 Rab8a

Get tips on using MicroRNA isolation kit to perform siRNA / miRNA gene silencing Rat - IEC-6 HuR