siRNA / miRNA gene silencing Rat RMC-1

Get tips on using Nrf2 siRNA (r) to perform siRNA / miRNA gene silencing Rat - Astrocytes Nrf2

Get tips on using Rn_LOC311846_1 FlexiTube siRNA to perform siRNA / miRNA gene silencing Rat - Astrocytes LRRC8A

Get tips on using Rn_Sod2_4 FlexiTube siRNA to perform siRNA / miRNA gene silencing Rat - PC12 SOD2

Get tips on using Rn_Rab7_5 FlexiTube siRNA(r) to perform siRNA / miRNA gene silencing Rat - NRVM( Rab7

Get tips on using HuR siRNA to perform siRNA / miRNA gene silencing Rat - IEC-6 HuR Lipid

Get tips on using Silencer® Select Negative Control No 1 siRNA to perform siRNA / miRNA gene silencing Human - siRNA negative control Lipid

Short hairpin or small hairpin RNA (shRNA) is artificial RNA, which has a hairpin loop structure, and uses inherent microRNA (miRNA) machinery to silence target gene expression. This is called RNA interference (RNAi). These can be delivered via plasmids or viral/bacterial vectors. Challenges in shRNA-mediated gene silencing include 1. Off-target silencing, 2. Packaging shRNA encoding lentivirus, and 3. Stable transduction in cells. RNAi has been designed to have anywhere from 19-27 bs, but the most effective design has 19 bp. In case commercial shRNAs are not available, potential target sites can be chosen within exon, 5’- or 3’ UTR, depending on which splice variants of the gene are desired. One should use the latest algorithms and choose at least two different sequences, targeting different regions, in order to have confidence in overcoming off-target effects. A BLAST search after selecting potential design will eliminate potential off-target sequences. For the second challenge, sequencing the vector using primers for either strand (50-100 bp upstream) is suggested, along with using enzymatic digestion on agarose gel for the vector. Next, once the shRNA-containing vector is packaged in a virus, it is important to check the viral titer before transduction. Finally, using a marker in the lentiviral vector (fluorescent protein or antibiotic resistance), along with qPCR for target gene expression can help in determining the efficacy of transduction and shRNA on its target site.

Get tips on using Rn_Pdcd8_1 FlexiTube siRNA to perform siRNA / miRNA gene silencing Rat - H9c2 AIF/Pdcd8

Get tips on using siRNA(r) NM_012820 Acsl1 to perform siRNA / miRNA gene silencing Rat - 3Y1 Acsl1

Get tips on using siRNA(r) NM_057107 Acsl3 to perform siRNA / miRNA gene silencing Rat - 3Y1 Acsl3