Wound healing assay cell type rat

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Get tips on using lentiCas9-Blast to perform CRISPR Mouse - Deletion RAW 264.7 Casp1

Products Addgene lentiCas9-Blast

Get tips on using Dusp3 siRNA to perform siRNA / miRNA gene silencing Mouse - RAW264.7 Dusp3

Products Thermo Fisher Scientific Dusp3 siRNA

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

RNA RNA sequencing Mouse RAW264.7

Get tips on using TruSeq Stranded Total RNA to perform RNA sequencing Mouse - RAW264.7

Products Illumina TruSeq Stranded Total RNA

Get tips on using pLKO.1 puro to perform CRISPR Mouse - Activation RAW 264.7 Dmd

Products Addgene pLKO.1 puro

Get tips on using Cytokeratin 19 Antibody to perform Immunohistochemistry Cytokeratin 19 - Rabbit Mouse -NA-

Products Novus Biologicals Cytokeratin 19 Antibody

Get tips on using MAGnify™ Chromatin Immunoprecipitation System to perform ChIP Mouse - RAW264.7

Products Thermo Fisher Scientific MAGnify™ Chromatin Immunoprecipitation System

Get tips on using Pierce™ Agarose ChIP Kit to perform ChIP Mouse - RAW264.7

Products Thermo Fisher Scientific Pierce™ Agarose ChIP Kit

Get tips on using Imprint® Chromatin Immunoprecipitation Kit to perform ChIP Mouse - RAW264.7

Products Sigma-Aldrich Imprint® Chromatin Immunoprecipitation Kit

Get tips on using CD98 siRNA (m) to perform siRNA / miRNA gene silencing Mouse - RAW264.7 CD98

Products Santa Cruz Biotechnology CD98 siRNA (m)

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