Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - SH-SY5Y
Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - BxPC-3
Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - Hep G2
Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - PANC-1
Get tips on using Muse™Autophagy LC3-antibody based Kit to perform Autophagy assay cell type - 143B
Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - AGS cell line
Get tips on using Muse® Cell Cycle Assay Kit to perform Cell cycle assay human - MDA-MB-231
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
As autophagy is a multi-step process which includes not just the formation of autophagosomes, but most importantly, flux through the entire system, including the degradation upon fusion with lysosomes, which makes it quite challenging for detection. There are several methods for detection in mammalian cells, including immunoblotting analysis of LC3 and p62 and detection of autophagosome formation/maturation by fluorescence microscopy, Currently, there is no single “gold standard” for determining the autophagic activity that is applicable in every experimental context, hence it is recommended to go for the combined use of multiple methods to accurately assess the autophagic activity in any given biological setting.
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