Isolating RNA from tissues and paraffin-embedded tissue samples can be challenging due to cross-linking of biomolecules and fragmented nucleic acids. The best solution is to slice the tissues into smaller pieces and make a homogenate solution (using tissue homogenizer or grinding liquid nitrogen frozen samples) in presence of RNAse inhibitors. The homogenization process should be carried out on dry ice to maintain the integrity of RNA.
Get tips on using RsaI (10 U/µL) to perform Restriction Enzymes RsaI / AfaI
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Rat Spleen
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Rat Lung
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Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Rat Liver
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Rat Heart
Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Tissue - Rat Brain
Get tips on using RNeasy Micro Kit to perform RNA isolation / purification Tissue - Rat Uterus
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