Protein Expression Prokaryotic cells M. smegmatis

Get tips on using EZ-Magna ChIP™ A/G Chromatin Immunoprecipitation Kit to perform ChIP Human - Fibroblast cell lines

Get tips on using OxiSelect™ Intracellular ROS Assay Kit (Green Fluorescence) to perform ROS assay cell type - MDA-MB-231

Get tips on using ROS-ID® Total ROS/Superoxide detection kit to perform ROS assay cell type - MDA-MB-231

The formation of DNA from an RNA template using reverse transcription leads to the formation of double-stranded complementary DNA or cDNA. The challenges with this process include 1. Maintaining the integrity of RNA, 2. Hairpin loops or other secondary structures formed by single-stranded RNA can also affect cDNA synthesis, and 3. DNA-RNA hybrids, which may result when the first strand of cDNA is formed. For the first challenge, using workflows that involve proper isolation and storage of RNA, and maintaining a nuclease-free environment helps obtain RNA with ideal 260/230 ratios. Using a reverse transcriptase that can tolerate high temperatures (50-55oC), overcomes obstacles imposed by secondary RNA structures. Finally, RNase H has the ability to hydrolyze RNA before the formation of a second cDNA strand. It is important to ensure that RNase H activity is optimal because higher RNase H activity leads to premature degradation of the RNA template. Many reverse transcriptases offer built-in RNase H activity.

Get tips on using Gibco™ DMEM, high glucose, GlutaMAX™ Supplement to perform 3D Cell Culture Media Mouse small intestinal organoids

Get tips on using Phospho-eIF2α (Ser51) (D9G8) XP® Rabbit mAb #3398 to perform Autophagy assay cell type - HEK 293

Get tips on using DMEM High Glucose w/ L-Glutamine w/o Sodium Pyruvate to perform Mammalian cell culture media U87

Get tips on using Gibco™ DMEM/F-12, GlutaMAX™ supplement to perform Stem cell culture media h-medial pallium induction and culture

Get tips on using anti-p62 / SQSTM1 (C-terminus) guinea pig polyclonal, serum to perform Autophagy assay cell type - MEFs (mouse embryonic fibroblasts)

Get tips on using RPMI-1640 with Phenol Red produced by FUJIFILM Wako Pure Chemical Corporation to perform Mammalian cell culture media Ku812