ChIP acH3 Rat Sheep BEF Tag

Get tips on using Ni-NTA HisSorb Strips (24) to perform Protein tag Detection of His-tagged proteins

Get tips on using Ni-NTA Spin Kit (50) to perform Protein tag Detection of His-tagged proteins

Get tips on using Penta·His Alexa Fluor 647 Conjugate to perform Protein tag Detection of His-tagged proteins

Get tips on using Ni-NTA Superflow (100 ml) to perform Protein tag Purification of His-tagged proteins

Get tips on using RGS·His Antibody, BSA-free (100ug) to perform Protein tag Detection of His-tagged proteins

Get tips on using Penta·His Alexa Fluor 488 Conjugate to perform Protein tag Detection of His-tagged proteins

Get tips on using Biotin Rat Anti-Mouse Ly-6A/E to perform Flow cytometry Anti-bodies Mouse - Ly-6A-E/Sca1

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

The RNA interference (RNAi) is used to inhibit gene expression or translation, by neutralizing targeted mRNA molecules. Two types of RNA molecules such as microRNA (miRNA) and small interfering RNA (siRNA) play a central role in RNAi. Few points have to considered to increase the transfection efficiency of siRNA. Always use healthy, actively dividing cells to maximize transfection efficiency. The confluency of cells should be between 50-70%. Always use the most appropriate siRNA concentration to avoid off-target effects and unwanted toxic side effects. Positive and negative controls should be used for each and every experiment to determine transfection efficiency.

Transfection is a powerful technique that enables the study of the function of genes and gene products in cells. Based on the nature of experiments, we may need a stable DNA transfection in cells for persistent gain-of-function or loss-of-function of the target gene. For stable transfection, integration of a DNA vector into the chromosome is crucial which requires selective screening and clonal isolation. By carefully selecting a viral delivery system and related reagents we can ensure safe and highly-efficient delivery of expression constructs for high-level constitutive or inducible expression in any mammalian cell type.