Immunohistochemistry Wilms Tumor 1 (WT1)

Get tips on using PCNA (D3H8P) XP® Rabbit mAb #13110 to perform Immunohistochemistry Mouse - PCNA

TUNEL assay is the cell death detection method where the biochemical marker of apoptosis is DNA fragmentation. The assay involves the microscopical detection of generated DNA fragments with free 3'-hydroxyl residues. in apoptotic cells using enzyme terminal deoxynucleotidyl transferase (TdT) which adds biotinylated nucleotides at the site of DNA breaks. Major challenges of this method involve proper access of the enzyme which could be hampered by poor permeabilization and/or excessive fixation with cross-linking fixative (common with archival tissue). This issue can be resolved by optimizing the incubation time with Proteinase K or CytoninTM.

Get tips on using Anti-vimentin to perform Immunohistochemistry Vimentin (3B4) - Mouse Human -NA-

Get tips on using Anti-MUC2 antibody (ab76774) to perform Immunohistochemistry Mouse - Muc-2

Get tips on using Mouse Osteopontin/OPN Antibody to perform Immunohistochemistry Mouse - Spp1/OPN

Get tips on using Pacific Blue™ anti-mouse Ly-6A/E (Sca-1) Antibody to perform Flow cytometry Anti-bodies Mouse - Ly-6A-E/Sca1

Get tips on using Notch1 (C44H11) Rabbit mAb #3268 to perform Immunohistochemistry Human - Notch1

Get tips on using Anti-p53 Protein, Clone DO7 to perform Immunohistochemistry Human - p53

Get tips on using 7ml p53 Bond RTU Primary to perform Immunohistochemistry Human - p53

Get tips on using Anti-Glutamine Synthetase antibody (ab73593) to perform Immunohistochemistry Rat - GS