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4 years ago
4 years ago by Ercole Udinesi
I have left my tumor samples in formalin for an extended period of time (around 10 weeks). Do you think I will be able to use them for TUNEL assay and get results? Thank you for your help in advance
Found 3 matching solutions for this experiment
|Warm slides to 57C for 5 minutes and treate with xylene and ethanol to deparaffinize
|Treat with Cytonin or Proteinase K and incubate at room temperature
Add Labeling Reaction Mix and incubate at 37 ˚C for 1 hour in a humidity chamber
|If no staining in TACS Nuclease, increase incubation time with Proteinase K (up to maximum 60 minutes) or Cytonin (up to overnight at 4 oC)
|Incubate tissue section for 30 min at +37°C with Proteinase K.
Add Tunel reaction and incubate for 60 min at +37°C in a humidified atmosphere in the
|To analyze use an excitation wavelength in the range of 450–500 nm and detection in the range of 515 – 565 nm (green).
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