siRNA / miRNA gene silencing Human HNSCC cell line

Get tips on using ROS-Glo™ H2O2 Assay to perform ROS assay cell type - HEK293

Get tips on using ROS-Glo™ H2O2 Assay to perform ROS assay cell type - HeLa

Get tips on using SQSTM1/p62 (D5E2) Rabbit mAb to perform Autophagy assay cell type - A2780

Get tips on using SQSTM1/p62 (D1Q5S) Rabbit mAb to perform Autophagy assay cell type - A549

Get tips on using LC3 Antibody (APG8B) (N-term) to perform Autophagy assay cell type - A549

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - HepG2

Get tips on using LC3 Antibody (APG8B) (N-term) to perform Autophagy assay cell type - HepG2

Get tips on using Annexin V-FITC Apoptosis Kit to perform Apoptosis assay cell type - K562

Get tips on using Autophagy/Cytotoxicity Dual Staining Kit to perform Autophagy assay cell type - Macrophages

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.