Protein Expression Eukaryotic cells CHO

Get tips on using CpoI (RsrII) restriction enzyme to perform Restriction Enzymes RsrII / CpoI

Get tips on using CpoI (RsrII) (10 U/µL) to perform Restriction Enzymes RsrII / CpoI

RNA-Seq is a method to sequence RNA by applying Next Generation Sequencing (NGS). The quality of RNA is critical for the success of RNA-Seq. The integrity of RNA is measured by the RNA integrity number (RIN). RIN is computed from RNA electrophoresis and electropherogram profiles (the peak area of the 28S rRNA should be approximately twice the peak area of the 18S rRNA). If you get the RIN value lower than 7, the possibility of getting the low quality of RNA-seq data is high. To get a high quality RNA, it is better to work with fresh samples or snap-freeze the tissues in liquid nitrogen as quickly as possible and store them at -80°C until further use. Make sure designated areas and all your filter tips, microfuge tubes, plastic, and glassware are RNase-free.

Get tips on using T4 DNA Ligase, Cloned to perform DNA ligation

Get tips on using Anti-NSE, Clone MIG-N3 to perform Immunohistochemistry Mouse - NSE

Get tips on using Anti-Calretinin Antibody, clone 6B8.2 to perform Immunohistochemistry Mouse - Calb2

Get tips on using Anti-CD133 Antibody, clone 13A4 to perform Immunohistochemistry Mouse - CD133

Get tips on using Anti-Somatostatin Antibody, clone YC7 to perform Immunohistochemistry Mouse - Somatostatin