Protein Expression Eukaryotic cells N. benthamiana

Get tips on using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) to perform

The DNA concentration after using this DNA isolation kit is sometimes too low and thus it is not sufficient for my follow-up experiments. How can I improve it?

Get tips on using DIA-310: Anti-CD31 (Ms) from Rat (Clone: SZ31) for mouse FFPE tissue to perform Immunohistochemistry CD31 - Rabbit Rat -NA-

Get tips on using Cellular ROS/Superoxide Detection Assay Kit to perform ROS assay cell type - Raw 264.7

How do I extract RNA from animal tissue without using liquid nitrogen? I tried the RNA extraction by using the TRIzol reagent and I homogenize the tissue using polytron homogenizer at room temperature for 30secs is this correct?

Get tips on using DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit to perform ROS assay cell type - mouse splenocytes

Get tips on using DCFDA / H2DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit to perform ROS assay cell type - C2C12

Get tips on using CellROX™ Deep Red Reagent, for oxidative stress detection to perform ROS assay cell type - U87

Get tips on using DCFDA - Cellular Reactive Oxygen Species Detection Assay Kit to perform ROS assay cell type - H9c2 rat cardiomyocytes

Get tips on using CellROX™ Deep Red Reagent, for oxidative stress detection to perform ROS assay cell type - mouse splenocytes