Microarray Gene expression arrays Human whole blood cells

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Get tips on using Gibco™StemPro™ Chondrogenesis Differentiation Kit to perform Stem cell Differentiation media hBMSCs differentiation into chondrogenic cells

Products Thermo Fisher Scientific Gibco™StemPro™ Chondrogenesis Differentiation Kit

Get tips on using X-tremeGENE™ HP DNA Transfection Reagent to perform DNA transfection Mammalian cells - Immortalized cell lines PANC-1

Products Sigma-Aldrich X-tremeGENE™ HP DNA Transfection Reagent

Get tips on using Gibco™ DMEM, high glucose, GlutaMAX™ Supplement to perform Stem cell culture media Mouse muscle satellite cells

Products Thermo Fisher Scientific Gibco™ DMEM, high glucose, GlutaMAX™ Supplement

Get tips on using MasterPure™ Complete DNA and RNA Purification Kit to perform DNA isolation / purification Cells - Immortalized cell lines C2C12

Products Epicentre MasterPure™ Complete DNA and RNA Purification Kit

Get tips on using Purified Mouse Anti-Nucleoporin p62 Clone 53/Nucleoporin p62 to perform Autophagy assay cell type - N27 dopaminergic cells

Products BD Biosciences Purified Mouse Anti-Nucleoporin p62 Clone 53/Nucleoporin p62

Get tips on using TaqMan® MicroRNA Reverse Transcription Kit to perform siRNA / RNAi /miRNA transfection Mouse - Glomerular Mesangial cells polymer / lipid

Products Thermo Fisher Scientific TaqMan® MicroRNA Reverse Transcription Kit

Get tips on using M-PER™ Mammalian Protein Extraction Reagent to perform Protein isolation Mammalian cells - SK-N-BE(2)-C

Products Thermo Fisher Scientific M-PER™ Mammalian Protein Extraction Reagent

I have tried to fabricate Liver organoids and would like to study the impact of FBS on healthy and tumor organoids. Since the compositions of FBS is unknown, do you recommend any alternatives like Human platelet lysate, etc?

Discussions Impact of using FBS

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

Cell culture media Mammalian cell culture media Vero

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

Cell culture media Mammalian cell culture media DH82

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