protein-expression-and-purification-mammalian-cells-hek-293-mt-pa

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Get tips on using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) to perform Cell cytotoxicity / Proliferation assay cell type - PC-3

Products Promega CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS)

Get tips on using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) to perform Cell cytotoxicity / Proliferation assay cell type - SH-SY5Y

Products Promega CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS)

Get tips on using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) to perform Cell cytotoxicity / Proliferation assay cell type - L-02

Products Promega CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS)

Get tips on using PowerPlex® 18D System to perform Cell line authentication Human iPSC cells derived from peripheral blood mononuclear cells

Products Promega PowerPlex® 18D System

Get tips on using TransIT®-LT1 Transfection Reagent to perform siRNA / RNAi /miRNA transfection Human Cells - Cal 27 cells Polymer / lipid

Products Mirus TransIT®-LT1 Transfection Reagent

Get tips on using Silencer® FANCD2 siRNA (human) to perform siRNA / miRNA gene silencing Human - 501 Mel and SK Mel 28 FANCD2

Products Dharmacon (GE Life Sciences) Silencer® FANCD2 siRNA (human)

I would like to excise a large strand of DNA and insert a new one using CRISPR. My problem is that my strand will be a little over 1kb and I am not sure if this is going to be a limiting factor. Also, how long should the homology arms be for a region of this size?

Discussions DNA insert using CRISPR

Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - Paneth cells

Products Thermo Fisher Scientific LC3B Antibody Kit for Autophagy

Get tips on using Cell Proliferation Kit I (MTT) to perform Cell cytotoxicity / Proliferation assay cell type - LTEP-a-2 lung adenocarcenoma

Products Sigma-Aldrich Cell Proliferation Kit I (MTT)

Get tips on using LC3B Antibody Kit for Autophagy to perform Autophagy assay cell type - Human neural progenitor cells (NPC)

Products Thermo Fisher Scientific LC3B Antibody Kit for Autophagy

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