rna-isolation-purification-cells-immortalized-bxpc-3

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Get tips on using LC3B (D11) XP® Rabbit mAb (Biotinylated) to perform Autophagy assay cell type - NIH-3T3

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Wound healing assay can be challenging due to inconsistencies and variations while making a wound on the confluent cell monolayer, consequently leads to wounds of varying sizes and widths. Moreover, this assay causes damage to the cells that are at the edge of the wound, which can prevent cell migration into the wound site and healing. The best solution is to use the standard wound healing assay kits using either combs or inserts to make a defined wound field or gap and prevent the well-to-well variation in these assays.

Get tips on using ON-TARGETplus Rat Rac1 (363875) siRNA - Set of 4 to perform siRNA / miRNA gene silencing Rat - MTLn3 Rac1

Get tips on using K-Ras Antibody (F234): sc-30 to perform Western blotting Ras

Get tips on using 53BP1 Antibody (H-300) rabbit polyclonal to perform Immunohistochemistry 53BP1 - Rabbit IgG Human -NA-

Get tips on using 53BP1 Antibody (H-300) to perform TissueFAxs 53BP1 [H-300] - Rabbit Human -NA-

Get tips on using 53BP1 Antibody (H-300) to perform Immunofluorscence  53BP1 [H-300] - Rabbit Human -NA-