Western blot 1,4 β-N-acetyl-D-glucosamine

Wound healing assay can be challenging due to inconsistencies and variations while making a wound on the confluent cell monolayer, consequently leads to wounds of varying sizes and widths. Moreover, this assay causes damage to the cells that are at the edge of the wound, which can prevent cell migration into the wound site and healing. The best solution is to use the standard wound healing assay kits using either combs or inserts to make a defined wound field or gap and prevent the well-to-well variation in these assays.

Get tips on using Monoclonal Mouse Anti-Human Ki-67 Antigen (Dako Omnis) Clone MIB-1 to perform Immunohistochemistry Human - Ki-67

Get tips on using EGMTM -2 MV Microvascular Endothelial Cell Growth Medium-2 BulletKitTM to perform Stem cell Differentiation media hMSCs differentiation into pericytes

Get tips on using pMT/BiP/V5-His A, B, & C Drosophila Expression Vectors to perform Protein expression and purification Insect cells - S2 HER2

Get tips on using ROS-ID® Total ROS/Superoxide detection kit to perform ROS assay cell type - A549 human adenocarcinomic human alveolar basal epithelial cells

Get tips on using Reactive Oxygen Species (ROS) Detection Assay Kit to perform ROS assay cell type - PLHC-1, SK-HEP-1, Hep3b, HepG2 human hepatocellular carcinoma

Get tips on using Prestained Dual Color Protein Molecular Weight Marker (1.7-40 kDa) Molecular Weight Marker to perform Protein Ladder Prestained

Get tips on using Monoclonal Mouse Anti-Human Cytokeratin 7 (Dako Omnis) Clone OV-TL 12/30 to perform Immunohistochemistry Human - CK7

Get tips on using APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU to perform DNA Damage Assay HeLa

Get tips on using DMEM/Ham's F-12 liquid medium w/o L-Glutamine to perform Stem cell culture media Human Tendon Stem/Pluripotence cells (TSPCs)