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Get tips on using ISOGEN to perform RNA isolation / purification Cells - immortalized CHO

DNA isolation and purification is the first critical step in sample preparation that helps ensure optimal performance of downstream assays like PCR, microarrays, and sequencing. Failure in yielding high-quality DNA would be the major reason that DNA doesn't work for the downstream application. To circumvent this, one should follow the recommended storage conditions to minimize DNA degradation by nucleases and shouldn't overload the purification system.

Get tips on using GenElute™ Mammalian Total RNA Miniprep Kit to perform RNA isolation / purification Cells - immortalized human pancreatic cancer

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized Raji

Get tips on using RNeasy Mini Kit to perform RNA isolation / purification Cells - immortalized DG-75

The biggest problem in isolating RNA from gram-positive bacteria is the disruption of the cell wall. A lot of protocols employ enzymatic digestion (pretreatment) which may affect gene expression patterns of certain genes. Therefore physical disruption using beads could be the best alternative.

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized SHG44

Get tips on using TRIzol Reagent to perform RNA isolation / purification Cells - immortalized LN15