shRNA gene silencing Mouse 4T1

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RNA siRNA / miRNA gene silencing Human Primary Human Aortic Endothelial Cells GLO-1 Lipid

DNA microarrays enable researchers to monitor the expression of thousands of genes simultaneously. However, the sensitivity, accuracy, specificity, and reproducibility are major challenges for this technology. Cross-hybridization, combination with splice variants, is a prime source for the discrepancies in differential gene expression calls among various microarray platforms. Removing (either from production or downstream bioinformatic analysis) and/or redesigning the microarray probes prone to cross-hybridization is a reasonable strategy to increase the hybridization specificity and hence, the accuracy of the microarray measurements.

DNA Microarray Gene expression arrays Mouse liver tissue Cyanine-3-CTP

RNA siRNA / miRNA gene silencing Human 501 Mel and SK Mel 28 FANCD2 Polymer / Lipid

RNA siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells hsa-miR-542-3p Lipid

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

DNA DNA methylation profiling Gene specific profiling Hypothalamus mouse tissue MeCP2

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

DNA DNA methylation profiling Gene specific profiling Mouse muscle stem cells SPRY1

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

DNA Site Directed Mutagenesis (SDM) Mouse Point mutation L929 SigmaR1 gene (σ1)

RNA siRNA / miRNA gene silencing Human Primary Endometrial Stromal Cells IGFBP1 (Insuline-like growth factor binding protein-1) Lipid
Cnp siRNA Product

Get tips on using Cnp siRNA to perform siRNA / miRNA gene silencing Mouse - BV2 CNPase

Products Thermo Fisher Scientific Cnp siRNA

Get tips on using Wwtr1 siRNA to perform siRNA / miRNA gene silencing Mouse - C2C12 Taz

Products Thermo Fisher Scientific Wwtr1 siRNA

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