siRNA / RNAi /miRNA transfection Human HeLa

Get tips on using siGENOME Human IKBKB (3551) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - PANC-1 IKKβ/IKBKB

Get tips on using siGENOME Human GSK3A (2931) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - PANC-1 GSK-3α

A gross majority of classical apoptotic attributes can be quantitatively examined by flow cytometry, the preferred platform for rapid assessment of multiple cellular attributes at a single-cell level. However, sample preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells. One of the major challenges is to make a specific construct that has no responses other than those related to the signaling pathway of interest. This can be achieved by selecting highly specific reporter constructs containing only defined responsive elements and a minimal promoter linked to reporter enzymes such as luciferase

Cell Invasion or Cell Migration assays are technically challenging to set up as the gradient between the two compartments equilibrates in time during the assay. It is also problematic to view cells and for cells to migrate through a non-physiologic polycarbonate or polypropylene filter. Care must be taken while loading the well with cells to form a single cell suspension. Precaution must be taken while trypsinization (under-trypsinization can lead to cell clumping while over-trypsinization could strip off adhesion molecules necessary for migration). This leads to difficulty in getting significant results, when only small numbers of cells cross the filter or when the distribution and/or staining of the cells is uneven.

Get tips on using ON-TARGETplus Human LRRC8A (56262) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - A2780 LRRC8A

Get tips on using ON-TARGETplus Human CASP3 (836) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - Huh7 CASP3

Get tips on using ON-TARGETplus Human RET (5979) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - TT RET

Get tips on using ON-TARGETplus Human EGFR (1956) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - TT EGFR

Get tips on using ON-TARGETplus Human KDR (3791) siRNA - SMARTpool to perform siRNA / miRNA gene silencing Human - TT KDR