siRNA / miRNA gene silencing Human HNSCC cell line Eph receptor B4

Get tips on using ROS-Glo™ H2O2 Assay to perform ROS assay cell type - HeLa

Get tips on using SQSTM1/p62 (D5E2) Rabbit mAb to perform Autophagy assay cell type - A2780

Get tips on using SQSTM1/p62 (D1Q5S) Rabbit mAb to perform Autophagy assay cell type - A549

Get tips on using LC3 Antibody (APG8B) (N-term) to perform Autophagy assay cell type - A549

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - HepG2

Get tips on using LC3 Antibody (APG8B) (N-term) to perform Autophagy assay cell type - HepG2

Get tips on using Annexin V-FITC Apoptosis Kit to perform Apoptosis assay cell type - K562

Get tips on using Autophagy/Cytotoxicity Dual Staining Kit to perform Autophagy assay cell type - Macrophages

The process of RNA extraction from bacteria, in general, involves an RNA-protective, effective lysis of bacterial cell wall (which may pose difficulties). EDTA promotes loss of outer membrane to provide lysozyme with access to peptidoglycan. Another common method for cell wall lysis is mechanical disruption using a homogenizer (applied for gram-positive bacteria and some strains of gram-negative bacteria). Following lysis, it is necessary to disrupt protein-nucleic acid interactions, which can be achieved by adding sodium dodecyl sulfate (SDS). Next step involves using phenol-chloroform-isoamyl alcohol extraction, where RNA can be obtained from the bottom organic phase, the top phase consists of DNA and the interphase contains proteins. Isoamyl alcohol is an inert and optional addition to this mixture and is added as an anti-foaming reagent to reduce the interphase. Following RNA extraction, the samples should be checked for its quality by gel electrophoresis (23S and 16S rRNAs and 5s rRNA and tRNA bands) or UV spectrophotometric or fluorescence methods.

Get tips on using Minimum Essential Medium Eagle to perform Stem cell Differentiation media hDPSCs differentiation into chondrogenic cells