DNA gel extraction / PCR product purification

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Get tips on using Corning® Hepatocyte Culture Media Kit, 500 mL to perform 3D Cell Culture Media Human prostate organoid

Products Corning Corning® Hepatocyte Culture Media Kit, 500 mL

Get tips on using MEBMTM Mammary Epithelial Cell Growth Basal Medium to perform 3D Cell Culture Media Mouse primary breast ephitelial cells-Mammospheres

Products Lonza MEBMTM Mammary Epithelial Cell Growth Basal Medium

Get tips on using MEBMTM Mammary Epithelial Cell Growth Basal Medium to perform 3D Cell Culture Media Human primary breast ephitelial cells-Mammospheres

Products Lonza MEBMTM Mammary Epithelial Cell Growth Basal Medium

Get tips on using pFastBac-GP67-H6HA1-His-RhPV-IRES-EGFP to perform Protein Expression Eukaryotic cells - S. frugiperda HA1 of H6N1 AIV

Products Rong-Huay Juang, Institute of Biotechnology, National Taiwan Uni pFastBac-GP67-H6HA1-His-RhPV-IRES-EGFP

Get tips on using Gibco™Advanced DMEM/F-12 to perform Stem cell Differentiation media hPSCs or iPSCs differentiation into Lung progenitor cells

Products Thermo Fisher Scientific Gibco™Advanced DMEM/F-12

Get tips on using QuikChange Site-Directed Mutagenesis Kit, 10 Rxn to perform Site Directed Mutagenesis (SDM) Human - Deletion HEK 293T BART promoter

Products Agilent Technologies QuikChange Site-Directed Mutagenesis Kit, 10 Rxn

Get tips on using In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based to perform Cell cytotoxicity / Proliferation assay cell type - THP-1

Products Sigma-Aldrich In Vitro Toxicology Assay Kit, Lactic Dehydrogenase based

Cells are sourced from various tissues to grow them in in-vitro conditions. Therefore, cell specific nutrients are important for their survival, maintenance and growth. Determining the appropriate cell culture media is a challenge if you are growing a cell line or a microorganism for the first time. Established cell lines, primary cells, stem cells, bacteria and Yeast all require varied nutrients from basic to complex. Based on the cell type, one can easy find what media and nutrients your peers have used before you try to reinvent the wheel.

Cell culture media Mammalian cell culture media PCAEC

Get tips on using Dulbecco’s Modified Eagle’s Medium/Ham’s Nutrient Mixture F12 to perform 3D Cell Culture Media PLC/PRF/5 cells-spheres

Products Sigma-Aldrich Dulbecco’s Modified Eagle’s Medium/Ham’s Nutrient Mixture F12

Get tips on using MEBMTM Mammary Epithelial Cell Growth Basal Medium to perform 3D Cell Culture Media Mouse primary breast cancer ephitelial cells-Mammospheres

Products Lonza MEBMTM Mammary Epithelial Cell Growth Basal Medium

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