siRNA / miRNA gene silencing Human PC3 (human prostate cancer cell line)

Get tips on using ROS-Glo™ H2O2 Assay to perform ROS assay cell type - HeLa

Get tips on using SQSTM1/p62 (D5E2) Rabbit mAb to perform Autophagy assay cell type - A2780

Get tips on using SQSTM1/p62 (D1Q5S) Rabbit mAb to perform Autophagy assay cell type - A549

Get tips on using LC3 Antibody (APG8B) (N-term) to perform Autophagy assay cell type - A549

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Autophagy assay cell type - HepG2

Get tips on using LC3 Antibody (APG8B) (N-term) to perform Autophagy assay cell type - HepG2

Get tips on using Annexin V-FITC Apoptosis Kit to perform Apoptosis assay cell type - K562

Get tips on using Autophagy/Cytotoxicity Dual Staining Kit to perform Autophagy assay cell type - Macrophages

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

ELISA is the most commonly used method of detecting and quantifying the concentration of an antigen in an unknown sample. During the experiment, If you get a weak signal, then make sure reagents are at room temperature before starting the assay. Try increasing incubation times to ensure maximal antibody binding and amplify the signal. Secondly, if you get values above 0 in the negative control indicates a high background signal. Try to consider reducing your antibody concentration and prevent non-specific binding of antibodies by using affinity-purified antibody and suitable blocking buffers. To avoid high well to well variation, do not stack plates during incubation, no bubbles in the plate and wash wells thoroughly to avoid variation.