rna-isolation-purification-cells-primary-rat-cortical-neurons

Hello everyone! I am going to do a live/dead assay for my cells and I saw that I can use both fluorescence and absorbance as my detection method. Is there a difference in the results depending on the method? Is one method preferred over the other in certain situations?

The estimation of DNA methylation level heavily depends on the complete conversion of non-methylated DNA cytosines. It is crucial to ensure complete conversion of non-methylated cytosines in DNA. Therefore, it is important to incorporate controls for bisulfite reactions, as well as to pay attention to the appearance of cytosines in non-CpG sites after sequencing, which is an indicator of incomplete conversion.

Get tips on using Radius™ 96-Well Cell Migration Assay to perform Cell migration / Invasion cell type - FaDu

Get tips on using Radius™ 24-Well Cell Migration Assay to perform Cell migration / Invasion cell type - SH-SY5Y

Get tips on using Beclin-1 (D40C5) Rabbit mAb to perform Cell cytotoxicity / Proliferation assay cell type - K562

Get tips on using Atg12 (D88H11) Rabbit mAb to perform Autophagy assay cell type - MT-2 (human T cell leukaemia)

Get tips on using Atg7 (D12B11) Rabbit mAb to perform Autophagy assay cell type - MT-2 (human T cell leukaemia)

Get tips on using Atg5 (D5F5U) Rabbit mAb to perform Autophagy assay cell type - MT-2 (human T cell leukaemia)

Get tips on using LC3A/B (D3U4C) XP® Rabbit mAb to perform Cell cytotoxicity / Proliferation assay cell type - K562

Get tips on using CytoTox 96® Non-Radioactive Cytotoxicity Assay to perform Cell cytotoxicity / Proliferation assay cell type - FADU