When extracting nucleic acids from cell cultures, thorough homogenization of cells via vortexing in lysis buffer is very necessary. Choose the best RNA isolation method keeping in mind the downstream applications, generally, column-based isolations result in clean and concentrated RNA samples. Downstream applications like sequencing and cDNA synthesis require high-quality RNA, always treat the samples with DNases and check their integrity by running a gel.
Get tips on using RNAqueous™ Total RNA Isolation Kit to perform RNA isolation / purification Cells - primary human lung fibroblasts
Get tips on using Qubit RNA HS Assay Kit to perform RNA quantification Fuorimetric - human blood
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Get tips on using Agilent RNA 6000 Pico Kit to perform RNA quantification Fuorimetric - human saliva
Get tips on using Ion Total RNA-Seq Kit v2 to perform RNA sequencing Rat - PC12
Get tips on using TruSeq RNA Library Prep Kit v2 to perform RNA sequencing Rat - PC12
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