rna-isolation-purification-cells-primary-rat-cardiac-fibroblasts

Get tips on using GABARAP (E1J4E) Rabbit mAb to perform Autophagy assay cell type - HEK 293

Get tips on using Atg16L1 (D6D5) Rabbit mAb to perform Autophagy assay cell type - HEK 293

Get tips on using mTOR (7C10) Rabbit mAb to perform Autophagy assay cell type - SH-SY5Y

Get tips on using Atg7 (D12B11) Rabbit mAb to perform Autophagy assay cell type - SH-SY5Y

Get tips on using Atg5 (D5G3) Rabbit mAb to perform Autophagy assay cell type - HLE-B3

Get tips on using Atg12 (D88H11) Rabbit mAb to perform Autophagy assay cell type - THP 1

Get tips on using Atg7 (D12B11) Rabbit mAb to perform Autophagy assay cell type - THP 1

Get tips on using Atg5 (D5F5U) Rabbit mAb to perform Autophagy assay cell type - THP 1

There are a plethora of detection methods of cell cytotoxicity and proliferation by flow cytometry. However, samples preparation for such flow cytometry-based techniques could be challenging. Cell harvesting by trypsinization, mechanical or enzymatic cell disaggregation from tissues, extensive centrifugation steps, may all lead to preferential loss of apoptotic cells. To overcome this strictly follow manufacturers instruction of the detection kit.

DNA ladder is typically used as a reference to estimate the size of unknown DNA samples that are separated based on their mobility in an electrical field. The critical points for running a DNA ladder are compatibility with running buffer, agarose gel percentage, and choosing the correct range of DNA ladder for sizing DNA molecules.