Protein Expression Prokaryotic cells L. citreum

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - mouse liver tissue

Get tips on using TRIzol Reagent to perform RNA isolation / purification Tissue - human lung tissue

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Rat - Liver tissue

Get tips on using TruSeq Stranded mRNA to perform RNA sequencing Mouse - 3T3-L1

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. Multiplexing such a reaction amplifies the design challenges where one target requires 3 primers, which should be exclusively bound nowhere in the template DNA or to each other. Similarly, two targets require 6, three require 9, and so on. Each amplicon needs to be either a different size (for gels) or labeled with a different fluorescent tag that is spectrally distinct from the others in the reaction. Further complicating this, different targets in the reaction can compete with each other for resources and causes more challenges in the detection of amplicons. However, with proper primer designing, their validation, optimize quality and concentration of the enzyme and buffers certainly lead to a successful multiplex PCR reaction.

A PCR reaction consists of the template DNA, two primers covering the amplification site, an enzyme, and buffers. Multiplexing such a reaction amplifies the design challenges where one target requires 3 primers, which should be exclusively bound nowhere in the template DNA or to each other. Similarly, two targets require 6, three require 9, and so on. Each amplicon needs to be either a different size (for gels) or labeled with a different fluorescent tag that is spectrally distinct from the others in the reaction. Further complicating this, different targets in the reaction can compete with each other for resources and causes more challenges in the detection of amplicons. However, with proper primer designing, their validation, optimize quality and concentration of the enzyme and buffers certainly lead to a successful multiplex PCR reaction.

Get tips on using Annexin-V-FLUOS Staining Kit to perform Necrosis LNCaP

Get tips on using Medium Range DNA Ruler to perform DNA Ladder Medium Range

Get tips on using 10 bp DNA Ruler to perform DNA Ladder 10 bp