siRNA / miRNA gene silencing Human 501 Mel and SK Mel 28 FANCD2

Site-directed mutagenesis (SDM) can be challenging, particularly during detection/confirmation of (SDM) in colonies by sequencing or PCR techniques. This common issue in SDM is heavily relying on designing of mutagenic primer pairs. The best solution is to design the mutagenic primers that have extended 3'-ends/3'-overhang. This would provide the annealing region between the mutagenic primer pair is essentially shorter. and hence ensure a lower annealing temperature for the primer pair along with a higher chance of annealing to the template.

Get tips on using EpiTect MethyLight PCR +ROX Vial Kit (200) to perform PCR Methylation specific PCR - Mammalian DNA

Get tips on using IntestiCult™ Organoid Growth Medium (Mouse) to perform 3D Cell Culture Media Mouse gastric cancer organoids

Get tips on using STEMdiff™ APEL™2 Medium to perform 3D Cell Culture Media hiPSC-derived kidney organoids

Get tips on using DMEM/F-12 PLUS Basal Medium to perform 3D Cell Culture Media BT-549 cells-Mammospheres

Get tips on using IntestiCult™ Organoid Growth Medium (Mouse) to perform 3D Cell Culture Media Mouse small intestinal organoids

Get tips on using DMEM (Dulbecco's Modified Eagle's medium) to perform Stem cell culture media Cord blood-derived endothelial cells(hCBiPS2)

Get tips on using Dulbecco’s Modified Eagle’s Medium - high glucose to perform Stem cell culture media Rat oligodendrocyte precursors (OPCs)

Get tips on using Gibco™ RPMI 1640 Medium to perform Stem cell Differentiation media hiPSCs differentiation into Microglial-like cells

Get tips on using Gibco™Neurobasal™ Medium to perform Stem cell Differentiation media hiPSCs differentiation into Microglial-like cells