Microarray Gene expression arrays A-375 human melanoma

DNA-protein interactions are studied by using ChIP. The basic steps in this technique are crosslinking, sonication, immunoprecipitation, and analysis of the immunoprecipitated DNA. During ChIP, if chromatin is under-fragmented or fragments are too large which can lead to the increased background and lower resolution. Shorter cross-linking times (5-10 min) and/or lower formaldehyde concentrations (<1%) may improve shearing efficiency. If Chromatin is over-fragmented, then optimize shearing conditions for each cell type to improve ChIP efficiency. Over-sonication of chromatin may disrupt chromatin integrity and denature antibody epitopes. If you do not see any product or very little product in the input PCR reactions, add 5–10 μg chromatin per IP.

Get tips on using Anti-REG1 alpha antibody (ab231092) to perform Immunohistochemistry Mouse - Reg1

Get tips on using Anti-Choline Acetyltransferase Antibody AB5042 to perform Immunohistochemistry Mouse - ChAT

Get tips on using Anti-activated Notch1 antibody (ab8925) to perform Western blotting Notch1

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Get tips on using APO-BrdU™ TUNEL Assay Kit, with Alexa Fluor™ 488 Anti-BrdU to perform DNA Damage Assay HeLa

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Get tips on using Anti-EpCAM antibody [323/A3] (ab85987) to perform Immunohistochemistry Mouse - EpCAM

Get tips on using Anti-Beclin 1 antibody (ab62557) to perform Autophagy assay cell type - RSC96

Get tips on using Cell-APOPercentage™ Apoptosis Assay to perform Apoptosis assay cell type - SKOV3